Why?
Startups and academic groups that operate in molecular biology, drug design, chemistry etc will at some point experience a need to produce a certain quantity of a specific protein for analysis (pull-downs, enzyme activity, cell penetration, small molecule interactions etc). The likelihood of any protein being available "of the shelf" from any vendor is fairly small increasing the cost of acquisition substantially. However, many druggable or otherwise therapeutically relevant proteins can be produced with fairly simple equipment available in most labs. Producing the proteins one self is, more often than not, faster and cheaper than getting it from vendors. In addition, shipping is avoided which minimises risks for problems with batch variation and sample integrity.
Procedure
After signing a digital non-disclosure agreement. I will give the protein in question a quick look and provide a quote. I will then go through literature and databases and provide an estimate for difficulty of in house production of the target. Should you decide to go ahead I will provide construct designs ready to order from a vendor of your choice, protocols and, if needed, a shopping list. Should you prefer, I will, also help evaluate and trouble shoot.
Timeframe
The initial evaluation takes about a day with construct designs and protocols about same. This does however depend on the desired analysis. Designs for structural work takes a bit longer.
Pricing
Initial evaluation is 3000 SEK (approx USD 300) VAT excluded, construct designs and protocols usually range between 5000 and 15000 SEK (approx USD 500-1500) VAT excluded.